Figure 2: Measurements of DPP4 promoter activity according to regulatory SNP g.-234A/C. The transcriptional activity of the DPP4 promoter genotype of g.-234A/C was analyzed by luciferase-based reporter assay as detailed in Materials and Methods. Reporter vectors containing -507 to -31 region of the DPP4 gene were cloned from homozygote with each of the genotype. Promoter activities were measured in Caco-2 cells (n=3) 2 days after transfection with the reporter plasmid. The reporter activity of each construct was compared with that of the control mock vector (pGL3-basic). Values are presented as means ± SD.