Figure 5: Endothelial cell (HUVEC) cytoskeletal organization as a function of culture duration, shear exposure magnitude and albumin additive, as measured by immunofluorescence microscopy. All data are reported as the mean SEM of independent experiments for(A,B) actin structure, (C,D)actin alignment and (E,F) connectivity. * Significantly different from no added albumin and paired BSA (paired by shear stress and culture duration, three-way ANOVA, P< 0.05), + Significantly different than 0.4Pa (paired by additive and culture duration, three-way ANOVA, P< 0.05), # Significantly different than early (paired by shear stress and additive, three-way ANOVA, P< 0.05).