Antibody used Manufacturer Dilution Secondary antibody
Rabbit anti Bax Santa Cruz Biotechnology Inc., USA (sc-526) 1:50 Biotinylated universal antibody raised in horse (Vectastain PK 6200; Vector Laboratories, Burlington, Ontario)
Rabbit anti P53 Santa Cruz Biotechnology Inc., USA (sc-6243) 1:50
Rabbit anti Caspase-3 Santa Cruz Biotechnology Inc., USA (sc-7148) 1:50
Table 1: Details of antibodies used for the immunohistochemical studies.