Sr. No. Dilution Factor Concentration (ppm) Total animals Mortality Mortality percentage
1 0 0 10 0 0%
2 200 5 10 0 0%
3 100 10 10 0 0%
4 50 20 10 0 0%
5 33.33 30 10 1 10%
6 20 50 10 1 10%
After 48 hours exposure period it was observed that there is 10% mortality in 30 ppm and 50 ppm concentrations of effluent sample
Table 3: 48 Hours Mortality.