Sr. No. Dilution Factor Concentration (ppm) Total animals Mortality Mortality percentage
1 0 0 10 0 0%
2 200 5 10 0 0%
3 100 10 10 1 10%
4 50 20 10 3 30%
5 33.33 30 10 4 40%
6 20 50 10 4 40%
After 72 hours exposure period it was observed that in 10ppm concentration of effluent there was 10% mortality, for 20ppm there was 30% mortality, 30ppm there was 40 % mortality and for 50 ppm there was 40% mortality observed
Table 4: 72 Hours Mortality.