Alpha value (for confidence interval) 0.05            
Probit Analysis - Finney Method [Lognormal Distribution]
Log10[Dose (Stimulus)] Actual Percent (%) Probit Percent(%) N R E(R) Difference Chi-square
0.699 0.025 0.0002 10 0.25 0.0021 0.2479 29.2063
1. 0.025 0.0025 10 0.25 0.0253 0.2247 2.
1.301 0.025 0.0187 10 0.25 0.1873 0.0627 0.021
1.4771 0.1 0.0487 10 1. 0.4867 0.5133 0.5414
1.699 0.1 0.1303 10 1. 1.3027 -0.3027 0.0704
Chi-square              
Chi-square 31.839            
Degrees Of Freedom 3            
p-level 0.            
Dose (Stimulus) Percentile    
Percentile Probit (Y) Log10[Dose (Stimulus)] Standard Error Dose (Stimulus) Standard Error    
1 2.6732 1.1986 -0.1425 15.7981 -5.2755    
5 3.3548 1.4824 -0.1189 30.3671 -8.4199    
10 3.7183 1.6338 -0.1149 43.028 -11.5156    
16 4.0056 1.7534 -0.0543 56.6736 -7.1028    
20 4.1585 1.8171 -0.077 65.6256 -11.7017    
25 4.3258 1.8867 -0.1355 77.0408 -24.4347    
30 4.476 1.9493 -0.1788 88.9726 -37.6666    
40 4.7471 2.0621 -0.2496 115.3785 -70.015    
50 5. 2.1674 -0.312 147.0423 -114.9488    
60 5.2529 2.2728 -0.3727 187.3957 -181.2693    
70 5.524 2.3856 -0.4366 243.0123 -287.5766    
75 5.6742 2.4482 -0.4717 280.6493 -368.3851    
80 5.8415 2.5178 -0.5106 329.4667 -482.9594    
84 5.9944 2.5815 -0.546 381.508 -616.3926    
90 6.2817 2.7011 -0.6123 502.4973 -967.5551    
95 6.6452 2.8525 -0.6957 712.0034 -1,694.882    
99 7.3268 3.1363 -0.8513 1,368.6063 -4,763.158    
Regression Statistics              
LC50 147.0423 LC50 Standard Error 221.9314        
LC50 LCL 13.9977 LC50 UCL 1,544.6442        
Log10[LC50] 2.1674 Standard Error 0.5211        
Beta 2.4016 Intercept -0.2054        
Beta Standard Error 1.9318            
Probit Analysis–Least squares [Normal Distribution]
Dose (Stimulus) Actual Percent (%) N Probit (Y) Weight (Z)
5. 0.025 10. 3.0396 1.0792
10. 0.025 10. 3.0396 1.0792
20. 0.025 10. 3.0396 1.0792
30. 0.1 10. 3.7183 2.6548
50. 0.1 10. 3.7183 2.6548
Regression Statistics        
LC50 119.6466 LC50 Standard Error 18.5731  
LC50 LCL 80.7726 LC50 UCL 158.5206  
Beta 0.017 Intercept 2.9629  
Beta Standard Error 0.021      
LC10 44.3663 LC16 60.9132  
LC84 178.38 LC90 194.9269  
LC100 207.7467      
Table 8: 48 Hours Probit analysis.