Figure 4: Exposure III: Gene induction caused by sediment-borne EE2. Foldchange of cyp19a1b (a) and vtg1 (b) from whole body homogenate of early juvenile zebrafish (23–25 dpf) exposed to sediment spiked with EE2. Fish (n = 3 pools) were sampled at the end of 3 d for Ref-Sed, Water-EE2 (150 ng L-1), Sed-1+Salt and Sed-1 and at the end of 5 d for Sed-2. Box-plots represent normalized gene expression expressed as fold-change relative to control or reference exposure (Ref-Sed). Note the expression levels are in log10 scale. Values are mean ± SD (15 fish per triplicate pool). Means with different letters are significantly different (P< 0.05) as determined by One-way ANOVA followed by Tukey’s HSD post hoc test.