| |
Method 1 |
Method 2 |
Method 3 |
Method 4 |
Method 5 |
| Conditioning of cartridges |
2×4 mL methanol
2×4 mL water |
5 mL methanol
5 mL water |
2×2.5 mL acetone
2×2.5 mL methanol
2×5 mL water |
3×2 mL hexane
2 mL acetone
3×2 mL methanol |
3 mL methanol
3 mL water |
pH of estrogens
standard solutions |
3 |
2 |
5 |
3 |
2 |
| Washing step |
2×5 mL methanol:water (1:1)
2×5 mL acetone:water (1:2) |
5 mL water |
25 mL hexane and water (1:4) |
5×2 mL water |
2×10 mL water |
|
Elution |
2×4.5 mL methanol |
10 mL acetonitrile |
2×10 mL acetone |
4 x1 mL acetone |
20 mL methanol |
| Complete dryness of solvents |
Vacuum |
Vacuum |
Vacuum |
Vacuum |
Vacuum |
| Redissolution of estrogens |
2 mL methanol |
2 mL acetonitrile |
2 mL methanol |
2 mL methanol |
2 mL methanol |
