Geneb Control Cd Zn Cd + Zn
abcb3 1.3 ± 0.4 1.0 ± 0.2 0.05 ± 0.01* 0.024 ± 0.005*
abcb3l1 1.1 ± 0.6 0.76 ± 0.14 0.10 ± 0.03* 0.09 ± 0.02*
abcb4 NDc 0.03 ± 0.01 0.017 ± 0.005 0.008 ± 0.002
abcb7 0.0013 ± 0.0011 0.003 ± 0.001 0.07 ± 0.03* 0.035 ± 0.015*
abcb8 0.0016 ± 0.0006 0.002 ± 0.001 0.007 ± 0.002* 0.008 ± 0.003*
abcb9 0.019 ± 0.009 0.004 ± 0.002 0.011 ± 0.003 0.008 ± 0.002
abcb10 0.38 ± 0.21 0.3 ± 0.1 0.012 ± 0.002* 0.013 ± 0.003*
abcb11b 0.02 ± 0.02 0.04 ± 0.02 0.008 ± 0.004 0.00046 ± 0.00009*
abcc1 1.4 ± 0.6 2.8 ± 1.1 0.11 ± 0.01* 0.08 ± 0.02*
abcc2 1.7 ± 0.4 0.53 ± 0.16* 0.020 ± 0.007* 0.007 ± 0.003*
abcc4 0.32 ± 0.16 0.18 ± 0.03 0.0201 ± 0.006* 0.014 ± 0.005*
abcc5 0.89 ± 0.20 0.27 ± 0.08* 0.10 ± 0.01* 0.086 ± 0.002*
abcc6a ND 0.011 ± 0.003 0.006 ± 0.001 0.0024 ± 0.0006
abcc7 ND 0.3 ± 0.1 0.005 ± 0.001 ND
abcc8 0.11 ± 0.06 0.05 ± 0.01 0.0019 ± 0.0006* 0.003 ± 0.001*
abcc9 4.2 ± 2.8 0.7 ± 0.5 1.7 ± 0.7 0.8 ± 0.1
abcc12 ND 0.004 ± 0.001 0.0025 ± 0.0007 0.0010 ± 0.0004
aRelative gene expressions (mean ± SEM, n = 5). bactin1 was the reference gene
bThe asterisk indicates a significant differential gene expression in zebrafish muscles, as determined with the Mann-Whitney U-test, *p < 0.05
cND: not detected. No Ct could be recorded or a Ct equal or above that of the negative control without DNA.
Table 2: abcb and abcc gene relative expressions in muscles after 7 days of metal exposurea.