Geneb Control Cd Zn Cd + Zn
abcb3 0.13 ± 0.03 0.59 ± 0.25 0.0095 ± 0.0021* 0.04 ± 0.01*
abcb3l1 0.15 ± 0.04 0.070 ± 0.026 0.072 ± 0.014 0.057 ± 0.026
abcb7 NDc ND (72 ± 31).10-4 ND
abcb8 (2.6 ± 1.5).10-5 (43 ± 14).10-5* (770 ± 240).10-5* (1520 ± 420).10-5*
abcb9 0.0019 ± 0.0009 0.0025 ± 0.001 0.0010 ± 0.0003 0.0017 ± 0.0009
abcb10 (86 ± 44).10-4 (340 ± 150).10-4 (87 ± 27).10-4 (170 ± 50).10-4
abcc1 0.008 ± 0.001 0.091 ± 0.025* 0.013 ± 0.005 0.014 ± 0.006
abcc2 0.054 ± 0.017 0.25 ± 0.10 27.10-4 ± 6.10-4* 0.027 ± 0.008
abcc4 0.019 ± 0.002 0.13 ± 0.03* 0.0053 ± 0.0008* 0.010 ± 0.001*
abcc5 0.17 ± 0.04 0.15 ± 0.03 0.032 ± 0.009* 0.040 ± 0.021*
abcc6a (89 ± 57).10-4 (95 ± 28).10-4 (9.4 ± 1.3).10-4 (4.1 ± 0.7).10-4
abcc8 (79 ± 44).10-5 (593 ± 118).10-5* (5.2 ± 1.2).10-5 (49 ± 21).10-5
abcc9 0.019 ± 0.006 0.03 ± 0.01 0.012 ± 0.004 0.05 ± 0.02
abcc12 ND ND ND (1.4 ± 0.4).10-4
aRelative gene expressions (mean ± SEM, n = 5). bactin1 was the reference gene
bThe asterisk indicates a significant differential gene expression in zebrafish gills, as determined with the Mann-Whitney U-test, *p < 0.05
cND: not detected. No Ct could be recorded or a Ct equal or above that of the negative control without DNA.
Table 3: abcb and abcc gene relative expressions in gills after 7 days of metal exposurea.