Geneb Control Cd Zn Cd + Zn
abcb3 0.79 ± 0.18 0.5 ± 0.2 0.21 ± 0.07* 0.31 ± 0.07*
abcb3l1 0.135 ± 0.005 0.49 ± 0.16* 0.24 ± 0.11 0.41 ± 0.24
abcb8 (21 ± 4).10-4 (20 ± 6).10-4 (14 ± 10).10-4 (41 ± 15).10-4
abcb9 0.0025 ± 0.0005 (366 ± 4).10-5* NDc 0.006 ± 0.006
abcc1 0.31 ± 0.015 0.35 ± 0.14 0.21 ± 0.07 0.37 ± 0.07
abcc2 0.72 ± 0.11 1.2 ± 0.2 0.7 ± 0.3 1.0 ± 0.3
abcc4 0.45 ± 0.15 0.4 ± 0.1 0.17 ± 0.11 0.45 ± 0.13
abcc5 (16 ± 3).10-5 (6 ± 4).10-5 (26 ± 26).10-5 (1.8 ± 0.6).10-5*
abcc8 0.35 ± 0.03 0.40 ± 0.04 0.3 ± 0.1 0.49 ± 0.16
abcc9 0.12 ± 0.02 0.07 ± 0.02 0.11 ± 0.01 0.1 ± 0.1
aRelative gene expressions (mean ± SEM, n = 5). bactin1 was the reference gene
bThe asterisk indicates a significant differential gene expression in zebrafish brain, as determined with the Mann-Whitney U-test, *p < 0.05
cND: not detected. No Ct could be recorded or a Ct equal or above that of the negative control without DNA.
Table 4: abcb and abcc gene relative expressions in brain after 7 days of metal exposurea.