Figure 3: Exposure to DEDTP decreased proliferation in T-CD8-positive cells. PBMCs were labeled with 1 μM CFSE, and stimulated with plate-bound anti- CD3/CD28 antibodies [1.5 μM/ml, each] in 24-well plates and treated with 50 μM DEDTP for 96 h. PBMCs were stained with anti-CD3-APC, and anti-CD8-PE (A). Histogram shows the main fluorescence index of CFSE of one representative experiment of four. The round division of T-CD8-positive cells was calculated by mitotic percentage and each division is represented as M (B). Mean ± SD (n=8), *p<0.05 by, ANOVA test.