Figure 4: DEDTP inhibits TCR/CD28-induced proliferation in T-CD8-positive cells by inducing cellular death. T-CD8-positive cells were stimulated with anti- CD3/CD28 antibodies [1.5 μM/ml, each] in 24-well plates and treated with 50 μM DEDTP for 24 (A), 48 (B), 72 (C), or 96 h (D). T-CD8-positive lymphocytes were stained with PI. Histograms show PI mean fluorescence index of one representative experiment of four. Mean ± SD (n=8), *p<0.05 by, ANOVA test.