Parameters Total of beta-lactams Total of sulphonamides
Calibration curve Y = -0125 + 0.7744 lnX Y = -21.758 ln (x) + 40.88
Regression coefficient 0.879 0.9926
Range of concentrations 0.00-58.9 ng / ml 0.125 to 5.0 ng / mL
IC50 (concentration at 50 % bounding) 0.65 ng / ml 0.75 ng / mL
Limit of detection (LOD) 1.5 μg / ml 13.3 μg / kg
Detection capability (CCβ) 48.5 μg / kg 54.8 μg / kg
Repeatability 5.05% 7.39%
Reproducibility (precision) 6.28% 8.82%
Recovery (accuracy) 96.18% 97.51%
Table1: Validation of parameters of the screening method for the analysis of betalactams & sulphonamides.