Figure 2: Micronucleus frequency (MN%) in tetrads of T. pallida exposed to different dilutions of vinasse NC. Negative control; PC. Positive control - MMS; RV. Raw vinasse; C1. vinasse diluted to 50%; C2. vinasse diluted to 25%; C3. vinasse diluted to 12.5%. When comparing the results from the first and second bioassays an increase in the frequency of micronuclei in T. pallida was observed for the vinasse crop 2 (Figure 2), although not statistically significant.