| Parameters |
Levels |
| Forward Extraction |
|
| AOT |
1 mM, 5 mM,10 mM,15 mM, 20 mM, 35mM |
| pH |
6.0, 6.5, 7.0, 7.5, 8.0, 8.5 |
| Time |
30 min |
| Temperature |
4°C |
| Backward Extraction |
|
| Salt Concentration |
1.5 M |
| pH |
8.0 |
| Time |
1 hr |
| Temperature |
4°C |
No. of replicates = 3
No. of runs = 108 |
