Time (h) Whole cotyledons Broken cotyledon
H-40 (mg A flask-1) H-50 (mg A flask-1) H-60 (mg A flask-1) H-40 (mg A flask-1) H-50 (mg A flask-1) H-60 (mg A flask-1)
Lupanine
0 453.43 ± 34.92 428.48 ± 10.37 487.72 ± 33.82 498.37 ± 36.13 557.58 ± 13.47 467.66 ± 4.84
24 313.29 ± 2.30 285.94 ± 19.83 271.21 ± 4.61 306.56 ± 19.51 273.85 ± 8.68 239.32 ± 6.93
48 189.42 ± 10.88 136.33 ± 0.35 133.19 ± 8.59 192.00 ± 23.79 123.37 ± 10.53 103.29 ± 5.60
Sparteine
0 203.07 ± 6.21 254.00 ± 2.64 225.19 ± 28.07 215.35 ± 8.41 192.63 ± 4.16 211.85 ± 6.38
24 155.91 ± 0.81 155.06 ± 11.26 167.42 ± 23.54 143.70 ± 0.25 137.42 ± 0.09 125.99 ± 0.44
48 130.09 ± 10.51 96.50 ± 0.17 83.21 ± 0.21 115.91 ± 2.35 62.04 ± 1.03 64.93 ± 4.78
Tetrahydrorombifoline
0 55.98 ± 1.04 51.17 ± 0.63 46.86 ± 2.96 51.05 ± 2.51 57.27 ± 1.41 55.75 ± 2.49
24 47.44 ± 3.37 28.53 ± 2.41 21.53 ± 0.66 41.68 ± 3.06 37.93 ± 1.70 28.54 ± 2.03
48 42.93 ± 4.45 18.12 ± 2.29 17.96 ± 1.62 31.60 ± 3.00 22.19 ± 2.00 17.66 ± 1.90
4-Hidroxylupanine
0 49.53 ± 1.66 42.11 ± 1.21 47.05 ± 3.29 40.89 ± 2.51 48.35 ± 1.37 46.53 ± 2.51
24 42.51 ± 2.67 35.39 ± 1.05 34.80 ± 2.99 33.07 ± 2.70 33.71 ± 2.73 30.21 ± 2.35
48 34.47 ± 1.48 25.86 ± 2.27 28.72 ± 2.65 26.88 ± 2.71 26.81 ± 2.29 23.78 ± 2.46
Minor alkaloids
0 89.51 ± 7.53 77.81 ± 3.33 80.06 ± 1.79 77.97 ± 5.92 76.64 ± 3.74 86.48 ± 0.73
24 71.00 ± 6.06 58.81 ± 3.59 58.47 ± 4.32 57.07 ± 5.98 55.14 ± 3.26 58.76 ± 2.52
48 54.08 ± 2.02 55.39 ± 3.77 53.86 ± 3.41 39.36 ± 3.51 49.16 ± 3.28 51.10 ± 2.00
Table 5: Quantity of each alkaloid present in the medium of fermentation during 48 h.