MZ = meso-zeaxanthin (highlighted in red); Z = zeaxanthin; L = lutein; Normal phase chiral conditions were used to obtain the above chromatographs. Absorbance spectra are shown for the MZ peak.
A, D, G and J = chromatography for non-saponified samples showing ester peaks obtained from salmon skin, sardine skin, trout skin, and trout flesh, respectively;
B, E, H and K = chromatography for saponified samples (5% KOH at room temperature overnight) showing MZ peaks obtained from salmon skin , sardine skin, trout skin, and trout flesh, respectively;
C, F, I and L = chromatography for saponified samples (5% KOH at room temperature overnight) co-eluted (spiked) with MZ standard showing increased MZ peaks obtained from salmon skin , sardine skin, trout skin, and trout flesh, respectively
Figure 2: Chromatography illustrating the presence of MZ in several fish species tested