| Carotenoid ratio % |
Standard (unsaponified) |
Mild saponification |
Strong saponification |
Intense saponification |
| MZ% |
0.6 |
0.5 |
0.5 |
5.5 |
| Z% |
7.6 |
6.2 |
6.9 |
7.7 |
| L% |
91.8 |
93.3 |
92.6 |
86.8 |
| L:MZ |
153 |
186.6 |
185.2 |
15.8 |
MZ = meso-zeaxanthin; Z = zeaxanthin; L = lutein; Carotenoid ratio % refers to
the percentage of each carotenoid in the standard. Standard (unsaponified) =
unsaponified lutein standard; Mild saponification = 5% KOH at room temperature
overnight; Strong saponification = 10% KOH at 45°C overnight; Intense
saponification = 10% KOH at 120°C overnight. Note: only following intense
saponification of the standard was L converted to MZ, reflected in an altered L:
MZ %ratio |
