Figure 1: HPLC profiles of pentapeptide (400 μg/mL) incorporated in water at pH 3.7, apple juice alone, and pentapeptide incorporated in apple juice stored at 0 (left) and 60 (right) days. Solvent system: 0.1% TFA in water and 0.1%TFA in water and acetonitrile (50:50); flow rate: 1 mL/min; injection volume: 10 μL; elution time: 45 min; absorbance measured: 215 nm; retention time of pentapeptide: 12.0 min.