Temperature Strain Y44 Strain Y46 60°C / 30 min 14.4 ±0.14 15.5 ±0.23 70°C/ 20 min 14.4 ±0.14 15.5 ±0.23 80°C/ 20 min 14.4 ±0.14 15.5 ±0.23 100°C/ 15 min 10.8 ±0.21 11.2 ±0.18 121°C/ 15 min - - pH 2 - - 4 14.1 ±0.29 15.2±0.09 6 14.3 ±0.17 15.1 ±0.15 8 12.6 ±0.22 13.4 ±0.35 10 - - Proteases a-chymotrypsine - - Trypsine - - Detergents Urea 10.9 ±0.17 11.9 ±0.21 Tween 80 19.25 ±0.23 20.37 ±0.16

Table 4: Inhibitory activity of neutralized supernatant of Leuconostoc strains Y44, Y46 under heat, pH, proteases and detergents effect.