Alu I.D. Forward Primer Reverse Primer + Alu - Alu Chr. # Yb8NBC65 5’-atctcatctccctgcctctg-3’ 5’-gggaggtctggagatctgtg-3’ 517 186 6 Yb8NBC109 5’-gtgcctggtactctagaaataaaactctct-3’ 5’-agaatgaaactccggctcaaa-3’ 561 251 22 Yb8NBC201 5’-ggagaaaatgtaaggtttctagcac-3’ 5’accaatgcaactatctacactgaca-3’ 476 145 7 Yb8NBC241 5’-ggactgtgtctaagggtgtcct-3’ 5’-ggtaattgggagcagttgaga-3’ 427 97 7

Table 1: Loci and primers utilized for the generation of Alutetraplex 3 from Carroll et al. [4]. Sizes (bp) are given for amplification products of the presence and absence alleles, as well as the chromosome that harbors the locus.