Figure 5: BBS10 silencing prevents FK-induced AQP2 accumulation at the apical plasma membrane in renal MCD4 cells. Both BBS1- and BBS10-silenced MCD4 cells were either left under resting condition or stimulated with FK. Biotinylated apical proteins were isolated and immunoblotted with anti-AQP2 antibodies. After normalization for total AQP2 amounts, apical AQP2 was quantified by densitometry. Experiments were repeated three times with similar results (** P<0.02).