Figure 6: BBS10 silencing is associated with misrouting of AQP2 at the basolateral membrane after FK stimulation in renal cells. Both BBS1- and BBS10- silenced MCD4 cells were either left under resting condition or stimulated with FK. Cells were subjected to immunofluorescence with anti-AQP2 antibodies and analyzed by confocal laser-scanning microscopy. Compared to controls cells (Figure 6A), BBS1 silencing did not affect the FK-induced accumulation of AQP2 at the apical pole in MCD4 cells (Figure 6B, white arrows). Conversely, BBS10 silencing prevented the apical translocation of AQP2 and clearly increased AQP2 staining at the basolateral plasma membrane (Figure 6C, white arrowheads). All images are representative of three independent experiments giving similar results.