Figure 2: Lentivector-enhanced expression of FPR in human marrow-derived mesenchymal stem cells. Panels A-D: Transduction of human marrow-derived mesenchymal stem cells with either HIV-CMV-EGFP or HIV-CMV-FPR-IRES2-EGFP. DIC Micrographs (A & C) and their corresponding fluorescent micrographs are displayed (B & D). Panel E: Chemotaxis assay was performed to determine the functionality of FPR expressed by the lentivector in MSCs. Open column indicates the level of chemotaxis of the MSCs expressing EGFP in response to 5 nM N-FP, while the closed column represents that of the MSCs expressing EGFP and FPR. Panels F-H: Flow cytometric analyses of the MSCs transduced with either HIV-CMV-EGFP (G) or HIV-CMV-FPR-IRES2-EGFP (H). The non-transduced MSC were also examined as shown (F).