Figure 1: Adenoviral gene transduction into small intestinal crypts in mice. Mice received AxCA-lacZ (2×108 pfu/mouse) infused either into general circulation through the tail vein (A) or into the intraluminal space of the small intestine (B and C). In order to remove mucus components of the epithelium, mice in C received Pronase MS® treatment prior to vector infusion, while mice in B received saline treatment (control). (A-C) β-galactosidase expression in the small intestinal epithelium. Numerous epithelial cells in the crypts were X-gal positive in C. (D) β-galactosidase activity in the small intestine after intraluminal AxCA-lacZ infusion. *p<0.05; n=6-8 in each group. (E) β-galactosidase expression of the livers of mice in C. No X-gal-positive cells were observed.