Figure 3: Growth of tumors derived from LSF-overexpressing B16F10 mouse melanoma clone in vivo. (a) Tumor growth in nude mice that were subcutaneously injected with LSF-overexpressing B16F10 or control clones. The mean volumes of tumors from five (control) and six (LSF-overexpressing) mice with standard deviation were plotted against days after injection. (b to i) Representative tumor in a nude mouse allografted with control B16F10 cells (b to e) and with LSF-overexpressing B16F10 cells (f to i) at 11 days after injection. HE staining of the tumors (c, g) and immunohistochemical detection of the proliferation marker Ki67 (d, h) and LSF (e, i). Scale bar, 100 μm. Growth of tumors derived from LSF-overexpressing B16F10 mouse melanoma clone in vivo. (a) Tumor growth in nude mice that were subcutaneously injected with LSF-overexpressing B16F10 or control clones. The mean volumes of tumors from five (control) and six (LSF-overexpressing) mice with standard deviation were plotted against days after injection. (b to i) Representative tumor in a nude mouse allografted with control B16F10 cells (b to e) and with LSF-overexpressing B16F10 cells (f to i) at 11 days after injection. HE staining of the tumors (c, g) and immunohistochemical detection of the proliferation marker Ki67 (d, h) and LSF (e, i). Scale bar, 100 μm.