Figure 6: Induction of p21 upregulation and growth suppression in mouse normal melanocytic cell lines overexpressing LSF. (a) Net proliferation of melan-a cells transiently transfected with the indicated gene-expression vectors was quantified by crystal violet staining (bottom) three days after transfection. p21 expression in melan-a cells transiently transfected with the indicated expression or empty vectors was examined by RT-qPCR (b) and immunoblotting (c). Bars represent mean ± SD of three independent experiments. **P<0.01; *P<0.05.