Figure 1: Effects of heterogeneous secretory signal peptides on fVIII expression. The original signal peptide of fVIII (BDD) was replaced with the various signal peptides listed in the table. The HMM score of each signal peptide is shown in column 3. All factor constructs were under the control of b-actin promoter with a CMV enhancer. At 24 hours post transfection into 293 cells, the secreted fVIII was collected and the coagulation activity was determined by aPTT assay.