| Antibody |
Clone |
Dilution |
Provider |
Retrieval method |
Positive score |
| ER |
SP1 |
Ready to use |
Ventana |
HIER pH 6 |
Allred score ≥ 3 |
| PR |
1E2 |
Ready to use |
Ventana |
HIER pH 6 |
Allred score ≥ 3 |
| HER2 |
4B5 |
Ready to use |
Ventana |
HIER pH 6 |
3+ |
| Ki-67 |
SP6 |
1/100 |
BioCare |
HIER pH 6 |
>14% |
| ANXA1 |
Clone29 |
1/500 |
BD |
HIER pH 9 |
0-4 low expression
6-12 high expression |
| FOXA1 |
Polyclonal |
1/500 |
Abcam |
HIER pH 6 |
0-4 low expression
6-12 high expression |
| VIM |
3B4 |
1/100 |
Dako |
HIER pH 6 |
0-4 low expression
5-12 high expression |
ER: Estrogen receptor; PR: Progesterone receptor; HER2: Human epidermal
growth factor receptor 2; ANXA1, Annexin A1; FOXA1: Forkhead box protein A1;
VIM: Vimentin; HIER: Heat-induced epitope retrieval |
