| Microscopic histopathology finding |
Experimental group/ Animal # |
| Vehicle (0.9- Saline) |
| 1 |
2 |
3 |
4 |
5 |
| Multi-focal tubular dilatation and basophilia (cortex) |
0 |
0 |
0 |
0 |
1 |
|
|
Doxorubicin(5 mg/kg/dose) |
|
21
|
22
|
23
|
24
|
25
|
|
Multi-focal decrease of Bowman’s space and mesangial expansion (glomeruli)
|
1
|
1
|
1
|
1
|
1
|
|
Multi-focal hyaline casts (intratubular)
|
2
|
2
|
1
|
2
|
2
|
|
Multi-focal tubular vacuolization with fine hyaline droplets (cortico-medullary junction)
|
1
|
1
|
1
|
1
|
1
|
| Focal tubular degeneration with individual cell necrosis (cortico-medullary junction) |
1
|
1
|
1
|
1
|
1
|
| Multi-focal tubular dilatation and basophilia (cortex) |
0
|
1
|
0
|
1
|
0
|
| Doxorubicin related renal histopathology severity scores were based on the classification of microscopic evidence of renal injury observed at termination on study day 14; and the distribution and increased numbers of renal cells affected were assigned a quantitative severity score: 0 = no abnormality noted, 1 = slight (minimal, <25%), 2 = mild (25-50%), 3 = moderate (>50%) and 4 = severe (>75%). On study days 1 and 8, male Sprague-Dawley rats were administered via a lateral tail vein a bolus intravenous injection of either doxorubicinor saline. |
