Cancer type Cell line EpCAM antibody EpCAMaptamer Pancreatic cancer PANC-1 ++ ++ ASPC-1 ++ ++ PANC-28 + + Breast cancer MDA-MB-231 ++ ++ MDA-MB-468 ++ ++ BT-474 + + SKBR3 ++ ++ Ovarian cancer OVCAR3 + + SKOV3 + + Hey + + Prostate cancer DU145 ++ ++ 22RV1 + + VCap + + PC3 + + Colon cancer HT29 ++ ++ Epithelial cells 293T ++ ++ HeLa + + Chronic myelogenous leukemia K562 ++ ++ Burkitt’s lymphoma CA46 - - Mantle Cell Lymphoma Jeko-1 - - Mantle Cell Lymphoma Maver-1 - - Acute Myeloid Leukemia HEL - - Histiocytic lymphoma U937 - -

Note: “++” EpCAM overexpression, “+” EpCAM -positive, “-” EpCAM-negative

Table 1: Summary of EpCAM aptamer binding to differentiate cancer cells.