No. Name Sequence No. Name Sequence No. Name Sequence No. Name Sequence 1 ZIC4-3 YKTSLVMRKRLRLYR 44 SIAH1-267 FAENGNLGINVTISM 87 MYBBP1A-1134 LYWQAMKTLGVQRPK 130 KIAA0391-180 KYLYLCVFHMQTSEV 2 ZIC4-185 FKAKYKLVNHIRVHT 45 TAS2R13-30 INCIDWVSKRELSSV 88 MYBBP1A-1306 IRSPSLLQSGAKKKA 131 KIAA0391-478 DDPFLLYATLHSGNH 3 ZIC4-269 RGCDKCYTHPSSLRK 46 TAS2R13-110 KIASFSSPAFLYLKW 89 ANK1-34 CFVLKHIHQELDKEL 132 SND1-423 INIAEALVSKGLATV 4 SDHB-238 FSLYRCHTIMNCTRT 47 TAS2R13-179 VKFTMTMFSLTPFTV 90 ANK1-99 TKKIIRKVVRQIDLS 133 PCDHA7-172 LSPNEYFFLDVPTSN 5 MGC17553-17 PSKENWFRQLRSQAV 48 TAS2R13-284 GNAKLRQAFLLVAAK 91 C15orf15-22 VRNDCKVFRFCKSKC 134 PCDHA7-789 PNPDWRYSASLRAGM 6 MGC23985-18 LTCYADDKPDKPDDK 49 FGF23-6 LRLWVCALCSVCSMS 92 PRKCH-130 YDHFVANCTLQFQEL 135 ZNF449-69 ILELLVLEQFLTILP 7 RARS2-2 ACGFRRAIACQLSRV 50 FGF23-40 IHLYTATARNSYHLQ 93 PRKCH-171 TLTGSFTEATLQRDR 136 PPAN-69 RMTLQLIKVQEGVGE 8 RARS2-179 GLLGTGFQLFGYEEK 51 FGF23-85 ITGVMSRRYLCMDFR 94 PRKCH-360 SRSTLRRQGKESSKE 137 AGPAT6-45 LYMKSLLKIFAWATL 9 RARS2-359 QMLKIMGYDWAERCQ 52 FGF23-131 QYHFLVSLGRAKRAF 95 ENG-98 VLSVNSSVFLHLQAL 138 AGPAT6-171 RYCFLLPLRIALAFT 10 RARS2-402 LRMLQNMASIKTTKE 53 TFAM-5 RSMWGVLSALGRSGA 96 ENG-473 SFVQVRVSPSVSEFL 139 AGPAT6-368 MVTYLLRMMTSWAIV 11 RARS2-500 QHLLRFDEVLYKSSQ 54 TFAM-38 LPRWFSSVLASCPKK 97 ENG-583 TSKGLVLPAVLGITF 140 CTRB1-229 GIVSWGSDTCSTSSP 12 IPO11-52 HTLDINVRWLAVLYF 55 TFAM-231 LRRTIKKQRKYGAEE 98 NOLA1-123 FYFSVKLSENMKASS 141 MAPK13-189 RAPEVILSWMHYNQT 13 IPO11-143 RQHRALLTFYHVTKT 56 CLDND1-12 ACVLSLISTIYMAAS 99 RPS15A-2 VRMNVLADALKSINN 142 ORC3L-212 SPPVVVILKDMESFA 14 IPO11-215 LKVLRKLTVNGFVEP 57 CLDND1-69 FRYNGTVGLWRRCIT 100 RPS15A-31 SKVIVRFLTVMMKHG 143 ORC3L-297 QFPFKINEKVLQVLT 15 IPO11-320 CMNLIKMIVKNYAYK 58 CLDND1-177 HLLAGLCTLGSVSCY 101 RPS15A-99 FGFIVLTTSAGIMDH 144 ORC3L-410 MNYFLVLRCLHKFTS 16 IPO11-526 DQDLVVRIETATTLK 59 MAFG-34 VRELNQHLRGLSKEE 102 C15orf15-23 RNDCKVFRFCKSKCH 145 MAP4K4-40 VEVVGNGTYGQVYKG 17 IPO11-579 HVLHVLSCVIERVNM 60 CCNG2-84 LDRFLALMKVKPKHL 103 RBMS3-130 PTNLYISNLPISMDE 146 MAP4K4-152 GLAHLHIHHVIHRDI 18 IPO11-708 KIINGYIFLSSTEFL 61 CCNG2-130 QCKCTASDIKRMEKI 104 CSNK1A1-13 VGGKYKLVRKIGSGS 147 RAPGEF4-44 PLRPANTITKVPSEK 19 SLC25A24-113 QSLQTLGLTISEQQA 62 CCNG2-181 SLDKLEAQLKACNCR 105 CSNK1A1-109 TMKTVLMLADQMISR 148 RAPGEF4-391 MMHCVFMPNTQLCPA 20 SLC25A24-248 RSLWRGNGTNVIKIA 63 CCNG2-231 KKHSKINDTEFFYWR 106 CSNK1A1-277 LRQLFRILFRTLNHQ 149 RAPGEF4-583 DVSVFTTLTINGRLF 21 SLC25A24-389 LGCGALSSTCGQLAS 64 CCNG2-270 WIVSRRTAQNLHNSY 107 C9orf32-156 SLRPNGIIVIKDNMA 150 RAPGEF4-680 QFWVVTEICLCSQLS 22 SLC25A24-430 LFRRIISKEGIPGLY 65 ACTL6B-146 FFLCKTAVLTAFANG 108 C9orf32-184 CRDLDVVRRIICSAG 151 RAPGEF4-841 SYVRQLNVIDNQRTL 23 SLC25A24-444 YRGITPNFMKVLPAV 66 ACTL6B-376 KLIASNSTMERKFSP 109 DKFZp762-2 LHSMSRLLSTKPSSI 152 SIRT1-26 MTLWQIVINILSEPP 24 OTX1-68 REEVALKINLPESRV 67 APEX1-168 VTAYVPNAGRGLVRL 110 DKFZp762-142 KWLISPVKIVSRPTI 153 SIRT1-246 VIGSSLKVRPVALIP 25 MKRN2-109 LRDRNLSGMAERKTQ 68 APEX1-189 DEAFRKFLKGLASRK 111 DKFZp762-365 PHFQGFQKLPSSPLG 154 VEGFD-155 NTSTSYISKQLFEIS 26 MKRN2-300 PSVYWVEDQNKKNEL 69 APEX1-250 ADSFRHLYPNTPYAY 112 SOSTDC1-9 YLLPLACILMKSCLA 155 VEGFD-173 TSVPELVPVKVANHT 27 MKRN2-367 QGTVRFFNSVRLWDF 70 APEX1-271 MNARSKNVGWRLDYF 113 SOSTDC1-156 KITVVTACKCKRYTR 156 ZNF187-274 CQKAFRLNSHLAQHV 28 MKRN2-402 GDLFMHLSGVESSEP 71 PARP15-9 FLHNIVVVSNCFYFQ 114 RNPC3-234 VLFGKPMVVQFARSA 157 TUFM-25 FLLQGLLRLLKAPAL 29 KCNS3-76 FRYVLNFYYTGKLHV 72 PARP15-382 YVVRVLTGVFTKGRA 115 CDC45L-15 QSQRVLLFVASDVDA 158 TUFM-408 KFNLILRQPMILEKG 30 KCNS3-171 IWIRMENPAYCLSAK 73 CLK1-75 EYRNDYTQGCEPGHR 116 CDC45L-196 TSSAMVMFELAWMLS 159 MIER3-359 NILNSFTASDLTALT 31 KCNS3-193 SVVLASIVAMCVHSM 74 CLK1-115 SKHRIHHSTSHRRSH 117 CDC45L-459 LFSRPASLSLLSKHL 160 MIER3-507 FISAHALHQHAALHS 32 KCNS3-240 RLAAAPCQKKFWKNP 75 KIF12-102 LYISRQTAQQMPSVD 118 CDC45L-488 LLPLVMAAPLSMEHG 161 ERp27-74 ILHSMVQKFPGVSFG 33 ATP6V0A1-128 LKFILRKTQQFFDEM 76 KIF12-203 CVSPSAQCLPETLST 119 ZNF649-480 QGKSPVNMVTVAMVA 162 ERp27-159 VIQIHLLLIMNKASP 34 ATP6V0A1-214 YVHKSVFIIFFQGDQ 77 KIF12-218 LRYASRAQRVTTRPQ 120 ABAT-370 FRPNAPYRIFNTWLG 163 C3orf37-169 DNWRLLTMAGIFDCW 35 ATP6V0A1-269 QMVLNQTEDHRQRVL 78 CTNND1-134 VRLLRKARDMDLTEV 121 ABAT-387 SKNLLLAEVINIIKR 164 HAPLN1-261 FYYLIHPTKLTYDVA 36 ATP6V0A1-297 VRKMKAIYHTLNLCN 79 CTNND1-211 LRNVSSERSEARRKL 122 ABAT-446 DDSIRNKLILIARNK 165 RNF32-29 LQLRNLSVADHSKTQ 37 ATP6V0A1-426 RESRILSQKNENEMF 80 CTNND1-480 NKSGNRSEKEVRAAA 123 CLIC5-41 ILWLKGVVFNVTTVD 166 RNF32-187 IKCVTRIQAYWRGCV 38 ATP6V0A1-494 LRGNPVLQLNPALPG 81 CTNND1-525 NNASRSQSSHSYDDS 124 CLIC5-212 TGLWRYLKNAYARDE 167 GLCE-17 CALFTLVTVLLWNKC 39 ATP6V0A1-526 TNKLTFLNSFKMKMS 82 TCF7L2-162 SNKVPVVQHPHHVHP 125 H2AFY-42 LWLKGVVFNVTTVDL 168 GLCE-504 PSSFVLNGFMYSLIG 40 ATP6V0A1-774 LFFFFTAFATLTVAI 83 TCF7L2-334 KEMRAKVVAECTLKE 126 H2AFY-214 LWRYLKNAYARDEFT 169 UXS1-134 VSDLVNGLVALMNSN 41 SIAH1-43 VCFDYVLPPILQCQS 84 MYBBP1A-251 LKMAASSVKKDRKLP 127 TOP3B-301 LNTVEMLRVASSSLG 170 ETV3-124 NYPFINIRSSGKIQT 42 SIAH1-182 QSCFGFHFMLVLEKQ 85 MYBBP1A-395 VRFLSPPALQGYVAW 128 TOP3B-628 HRFMKYIQAKPSRLH   43 SIAH1-211 LIGTRKQAENFAYRL 86 MYBBP1A-2036 KTLSMREVRSCFEDP 129 KIAA0391-4 YLFGIRSFPKLWKSP

Table 2: List of amino acid sequences of synthetic peptides used for the second screening. A total of 170 peptides were predicted as epitopes derived from 67 antigen proteins. The selected useful antigen peptides are shown in bold. Numbers of peptide names represent the first amino acid number of the original proteins.