Figure 3: Isolation of bacteriophage targets using immobilized heterofunctional thioredoxin fusion proteins. Thioredoxin acts as a solubilizer and allows the complex to bind to the column matrix in aqueous solution. After cleavage of thioredoxin, cell lysate is added to let the phage display derived peptide capture target antigens, which are eluted from the column by protease cleavage.