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Figure 1: (A) PCR amplification of CASP9 (–712C>T) showing amplified size of 194 bp (P1–P6) (B) HaeII restriction digestion of the PCR product with CASP9 (–712C>T) polymorphic sites: P1, P6–homozygous CC genotype, P2, P5–homozygous TT genotype, P3, P4– heterozygous CT genotype and NC–Negative control. L–100 bp molecular weight marker.*20 bp fragment not visible due to overrun. |