Figure 2: SDS-PAGE of the recombinant enzyme. A. Showing recombinant enzyme accumulation at different induction times. 4 µl of the cultivation culture supernatant was loaded in each lane. Lane M is BIO-RAD protein marker (Catalog #161-0373). Lane 1 is culture supernatant before induction. Lane 2 to lane 9 show 24-108 hours induction time as indicated (12 hours interval for each lane). B. Showing no enzyme accumulation at different induction times with P. pastoris of pPIC9 transformed. 4 µl of the cultivation culture supernatant was loaded in each lane. Lane M is BIO-RAD protein marker (Catalog #161-0373). Lane 1 is culture supernatant before induction. Lane 2 to lane 9 show 24-108 hours induction time as indicated (12 hours interval for each lane). C. Deglycosylation of the FPLC purified recombinant enzyme. Lane M is BIO-RAD protein marker (Catalog No.#161-0373). Lane 1 is 1µl of purified recombinant enzyme (108 hour). Lane 2 is 1µl of purified recombinant enzyme (108 hour) treated by Endo H.