Figure 1: a) Cells collected from a 48 h slant, ressuspended in PBS and stained with PI. One major population exists (A), corresponding to cells with intact cytoplasmic membrane, no staining.

(A) R. gluinis cells collected from a 48 h slant, ressuspended in PBS and killed in a water bath at 100ºC for 10 min and stained with PI. One main subpopulation (B) could be distinguished, corresponding to cells with permeabilised cytoplasmic membrane.