Figure 4: PI fluorescence density plots of R. glutinis CCMI 145 cells growing on a fed-batch fermentation taken at t=14.5 h (a), 20.3 h (d) , 62.2 h (g) and 118h (j). Subpopulation A corresponds to cells with intact membrane, no staining (PI-), while subpopulation (B) corresponds to cells with permeabilised membrane, stained with PI (PI+). PI fluorescence versus side scatter light (SS) [b), e), h) and k)] and forward scatter light [c), f), i) and j)] density plots for R. glutinis CCMI 145 cells growing on a fed-batch fermentation taken at t= 14.5 h, 20.3 h, 62.2 h , and 118 h, respectively. Subpopulation (C) corresponds to permeabilised cells (PI+), depicting lower SS signal compared to the SS signal of subpopulation (E) composed of intact cells (PI-). Subpopulation (D) corresponds to permeabilised cells (PI+) displaying the same SS signal as subpopulation (E). Subpopulation (G) corresponds to permeabilised cells (PI+) depicting lower FS signal compared to the FS signal of subpopulation (I) composed of intact cells (PI-). Subpopulation (H) corresponds to permeabilised cells (PI+) displaying the same FS signal as subpopulation (I).