Variables Coded setting levels
  -1 0 +1
Xylan (g/L) 5 10 15
Yeast Extract (g/L) 1 2 3
MgSO4.7H2O (g/L) 0.1 0.3 0.5
(NH4)2SO4 (g/L) 0 2.2 4.4
Peptone (g/L) 1 2 3
pH 5 6 7
Temperature (°C) 25 30 35
Table 2: Process variables used in the Plackett & Burman design, showing the treatment combinations.