Treatment Coded setting levels Real levels Xylanase activity (IU/mL)
X1 X2 X1 X2 LEB-AY10
1 -1 -1 13 26.5 3.600
2 1 -1 27 26.5 3.233
3 -1 1 13 33.5 1.267
4 1 1 27 33.5 1.367
5 -1.41 0 10 30 1.817
6 1.41 0 30 30 3.067
7 0 -1.41 20 25 2.033
8 0 1.41 20 35 1.400
9 0 0 20 30 10.417
10 0 0 20 30 11.250
11 0 0 20 30 10.833
Results represent the mean of three experiments.
X1 = xylan (g/L), X2 = T(°C), code settings are X1 = (xylan – 20)/7,
X2 = (temperature– 30)/3.5.
Table 3: Process variables used in the second CCD, showing the treatment combinations and the mean experimental response.