Figure 1: Relative quantity of adiponectin mRNA in liver, hypothalamus, and muscle of 6-wk-old male and female broiler chickens: Total RNA from each tissue was DNAse-treated, reverse transcribed and subjected to real-time quantitative PCR (QPCR) as described in materials and methods. Samples were run in triplicate and the average threshold cycle (Ct) values were determined for adiponectin and housekeeping genes (18S and β-actin). Relative quantity of adiponectin mRNA was determined by the 2-ΔΔCt method [67]. Data are presented as mean ± SEM (n = 3 for each gender and each tissue) and * represent significant difference at P<0.05.