Figure 3: Effect of leptin treatment on adiponectin gene expression in broiler chickens: Two homogenous weight-matched groups of 3-wk-old broiler chickens (n=5) were continuously treated with recombinant chicken leptin or saline solution. Birds were killed and total RNA was isolated from liver, hypothalamus, and muscle and subjected to RT-QPCR. Data are presented as mean ± SEM. * P<0.05 indicates a significant difference between leptin-treated group and the control.