Figure 5: (A): Molecular weight of purified L-asparaginase from Streptomyces radiopugnans MS1 SDS PAGE on gel of stained with Bio-Safe™ Coomassie stain. 1.Biorad (Protein) low range Protein Marker,2.Crude extract,3.Ammonium sulphate precipitate ,4.DEAE Column chromatography,5.Sephadex G-200 ,6.Prep cell 491 (B): Molecular weight of purified L-asparaginase from Streptomyces radiopugnans MS1 Native PAGE on gel of stained with Bio-Safe™ Coomassie stain.1,Biorad (protein)High range marker; 2,3 and 4 purified L-asparaginase. (C): Bio-Safe™Coomassie stained IEF strip containing purified L-asparaginase for determination of pI and evaluated with IEF standards (Bio-Rad, Hercules, CA ). (D): Representative 2D gels of Streptomyces radiopugnans MS1 protein sample stained with Bio-Safe™Coomassie stain.The standards (2.5 µl) were run on 7 cm ReadyStrips in the Mini-PROTEAN II CELL. The polypeptide molecular mass scale compare with 2-D SDS –PAGE Standards(Bio-Rad, Hercules, CA )in kDa is depicted on the y-axis while the x-axis on the pI range. The proteins were resolved in 4–7 linear pH gradient.