D-glucose (%w/v) Antibacterial activity (inhibition zone in mm)
  Bacillus pumilus Aeromonas formicans 
1.0 14.5 ± 0.50 19.0 ± 0.50
2.0 16.5 ± 0.50 19.8 ± 0.76
3.0 14.3 ± 0.58 15.7 ± 0.29
4.0 12.8 ± 0.76 15.2 ± 0.76
5.0 12.7 ± 0.58 14.3 ± 0.58
6.0 10.5 ± 0.50 12.5 ± 0.50
7.0 8.8 ± 0.76 11.5 ± 0.50
8.0 8.5 ± 0.50 9.80 ± 0.29
Table 4: Determination of optimum concentration of D-glucose for antibiotic production by cup-plate method.