Incubation time (days) Antibacterial activity (inhibition zone in mm)
  Bacillus pumilus Aeromonas formicans 
1 - -
2 8.2 ± 0.76 8.6 ± 0.29
3 14.6 ± 0.29 15.2 ± 0.50
4 27.5 ± 0.50 26.5 ± 0.50
5 22.0 ± 1.00 23.5 ± 0.58
6 19.6 ± 0.76 20.6 ± 0.50
7 17.3 ± 0.50 18.2 ± 0.29
8 16.9 ± 1.04 16.8 ± 0.76
Table 9: Determination of optimum incubation time for antibacterial production by cup-plate method.