Extraction method Number of detected peaksa in polar phase Number of detected peaksa in non-polar phase
Methanol 299 No phase separation
Biphasic-neutral 210 151
Biphasic-acidic 282 190
Biphasic-basic 216 251
aThe total peak number with the peak height cut off at 12,000.
Table 2: Comparison of total detected peak numbers and the yields of intracellular metabolites extracted from different solvent systems.