Figure 2: Immunoblotting and densitometric analysis of phosphorylated (p-ERK1/2) and total ERK1/2 protein in HCT-8 cells infected by G. intestinalis after 20 min of incubation. CTR, control cells; G, G. intestinalis-infected cells; G+PD98059, after pre-treatment with the specific inhibitor of ERK1/2 protein; ACT, actinomycin D-treated cells. Results are expressed as mean ± SD of five different experiments. The protein expression levels were normalized to those of β-actin and are expressed as means ± SD of five independent experiments. *P < 0.05 compared to CTR, **P < 0.01 compared to CTR.