Figure 4: Immunoblotting and densitometric analysis of phosphorylated (pJNK1/2) and total unphosphorylated (JNK1/2) protein in HCT-8 cells infected by G. intestinalis after 60 min of incubation. CTR, control cells; G, G. intestinalis-infected cells; G+SP600125, after pre-treatment with the specific inhibitor of JNK1/2; ACT, actinomycin D-treated cells. Results are expressed as mean ± SD of five different experiments. The protein expression levels were normalized to those of β-actin and are expressed as means ± SD of five independent experiments. *P < 0.05 compared to CTR, **P < 0.01 compared to CTR.