| Component |
Chemical
Formula |
Quantity
(per L distilled water) |
Stock Solution Preparation
(g/L distilled water) |
| MOPS (Organic buffer) |
C7H15NO4S |
1.9 g |
|
| Salt Solution |
|
|
|
| Sodium Chloride |
NaCl |
9.0 mL |
10.0 |
| Calcium Chloride |
CaCl2 |
9.0 mL |
10.0 |
| Magnesium Sulfate |
MgSO4 |
0.4 g |
|
| Ferrous Sulfate Solution (0.2%) |
FeSO4·7H20 |
4.5 mL |
2.0 |
| Trace Mineral Solution |
|
|
|
| Zinc Sulfate |
ZnSO4·7H20 |
4.5 mL |
0.880 |
| Cupric Sulfate |
CuSO4·5H20 |
4.5 mL |
0.039 |
| Manganese Sulfate |
MnSO4·4H20 |
4.5 mL |
0.0061 |
| Boric Acid |
H3BO3 |
4.5 mL |
0.0057 |
| Ammonium Molybdate |
(NH4)6Mo7O24·4H20 |
4.5 mL |
0.0037 |
| Isoleucine (Amino acids) |
C6H13NO2 |
7.8 g |
|
| Glucose |
C6H12O6 |
20.0 mL |
5.94 |
| Sodium Phosphate Monobasic Solution |
NaH2PO4·H2O |
5.5 mL |
1.24 |
|
