Parameter Growth in MYM brotha Acclimatization in production mediumb After ethanol shock During jadomycin productionc OD600 1.750 0.823 0.823 1.444 CFU/mL 2.25 x108 0.9x108 0.9 x108 2.67 x107 TF (µmol) 5.5 0.225 0.250 0.135 TF (µmol/CFU) 2.44x10-8 0.25 x10-8 0.28 x10-8 3.68 x10-8 Hyphae width (µm) 0.5-1 0.3-0.7 0.3-0.7 0.3-0.5 Cross walls √ √ √ x Branching x √ √ √ Budding x x √ √ Pinching x x √ √ Vesicles Large, numerous Fewer Fewer Fewer Spore septation ? √ √ √ Germinating spores x x ? √ aAfter 40 hours of growth in growth media bAfter 3 hours of acclimatization in production medium cAfter 48 hours of jadomycin production

Table 4: Changing in activity, morphology and structure of S. venezuelae during the various stages of growth and jadomycin production.